Histology Course Department of Pathology, University of Zurich Histology Course

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Conventional-histological Staining Immunohistochemistry (IHC) In Situ Hybridisation (ISH)


m: monoclonal antibody
p: polyclonal antibody

Antigen, binding to the antibody Stained structure/detection
CD20 m Cluster of Differentiation 20. Membrane-phosphoprotein 35/37 kDa (mAb L26 recognizes an intracytoplasmatic domain. Function: Regulation of cell activation (via calcium-ion-channel) Early B-cell-antigen, expressed up to the state of the mature plasma cell
CIV 22 m Type IV Collagen (basal lamina collagen) Basal lamina
Crg A m

Chromogranin A
Acidic sulfated glycoprotein found in secretory granules of most endocrine and neuroendocrine cells.

cytoplasm of endocrine cells
Desmin m

Polypeptide, MW 51.000

intermediate filament of muscle cells
GFAP p Glial fibrillary acidic protein
Polypeptide, MW 50.000
intermediate filament protein of astrocytes
hCG-beta m Human chorionic gonadotropin, hormonspecific subunit, glycoprotein, 145 amino acids
Intact hormone: MW ca. 37.000
cytotrophoblast, chorial giant cells in chorionepithelioma
HPV m Human Papilloma Virus
envelope protein
affected nuclei, possibly virus-antigen in cytoplasm
Ig m Immunoglobulin
heavy chain, type A, D, G or M
B-lymphocytes, plasma cells
proof of monoclonality possible
light chains m light chains of immunoglobulins kappa or lambda

B-lymphocytes, plasma cells
proof of monoclonality possible

CD 45 m Cluster of Differentiation 45. LCA: Leukocyte Common Antigen. Membran-Glycoprotein, 4 isoforms: 220, 205, 90, 180 kDa, with different expression on all leucocytes (mAbs LC recognizes all isoforms). Function in signal transmission Antigens on B- and T-cells, as well as monocytes, histiocytes and granulocytes
LU-5 m conformation-dependent epitope in all presently known 20 cytokeratins (polypeptides), MW 40.000 - 65.000 cells with cytokeratin-containing intermediate filaments: "epithelial and mesothelial cells"
PRL m Prolactin; one chained proteohormone, 198 amino acids, MW ca. 26.400 Prolactin-cells of hypophysis respectively of pituitary tumors
PSA m Prostate-specific Antigen
Glycoprotein, MW ca. 33.000
epithelial cells of prostate respectiveley of carcinoma of the prostate
Tg m Thyroglobulin
Glycoprotein, MW ca. 660.000
follicular epithelial cells of the thyroid, follicular and papillary thyroid carcinoma
S-100 p cytoplasmatic protein S-100
MW ca. 21.000
S-100 a: a- und b-subunits
S-100 b: 2 identical b-subunits
Antiserum recognizes epitopes on both subunits
cytoplasm of glial cells, Schwann's cells, satellite cells of the adrenal medulla, myoepithelial cells, melanocytes, melanoma cells, chondrocytes, dendritic reticulum cells et al.
SP m Substance P, peptide (Tachykinin)
11 amino acids, MW 1348
Substance P-ergic neurons, certain enterochromaffin cells, cells of ileum carcinoids
Syn p,m Synaptophysin, glycosylated polypeptide of membranes of presynaptic vesicles, MW 38.000 neuroendocrine cells, neuroendocrine tumor cells
Vi m Vimentin
Polypeptide, MW 55000
Vimentin-containing intermediate filaments of mesenchymal cells
a-synuclein An immunohistochemical staining to a-synuclein. Aggregated a-synuclein proteins form brain lesions that are hallmarks of some neurodegenerative diseases (so-called synucleinopathies, such as Parkinson's disease (PD) or dementia with Lewy bodies (DLB))
PrP An immunohistochemical staining to detect human prion protein (PrP). The antibody recognizes both the cellular (PrPC) and the pathological (PrPSc) form of PrP
A-b-amyloid An immunohistochemical staining detecting pathological Ab amyloid. Accumulates as extracellular Ab plaques (either as "soft" or "core" plaques), mainly in cortical areas of patients with Alzheimer's disease. In addition, Ab may accumulate within cerebral blood vessels resulting in a disease called cerebral amyloid angiopathy (CAA).
MAP2C An immunohistochemical staining using the antibody clone MAP2C detecting the microtubule-associated protein-2 (MAP-2). MAP-2 is concentrated in neuronal somata and dendrites.

also detects:
(i) oligodendroglial tumour cells with a perinuclear staining pattern
(ii) astrocytic tumour cells with a fibrillary staining pattern
Pancytokeratin cytoskeletal filaments

all epithelial structures