Histology Course A IV 54 Department of Pathology, University of Zurich Histokurs

A IV 54 follicular lymphoma, diffuse pattern (WHO 2001) HE  
  ICD-10: C83.9; ICD-O: 9691/3
48-year-old female underwent excision of supraclavicular lymph nodes.
Several sections of lymph nodes are shown which exhibit complete destruction of the regular architecture. The lymph nodes are diffusely permeated by centrocytes (small to medium-sized cells with angulated nuclei) and fewer centoblasts (large transformed cells with round to oval nuclei, vesicular chromatin, exhibiting one to three nucleoli).
Diffuse follicle centre lymphoma is composed of centrocytes and centroblasts and is thus considered a variant of follicular lymphoma (compare slide A_IV_53 ). In contrast to the latter, no follicles are formed. To diagnose diffuse follicle centre lymphoma the following criteria have to be met: (1) both, small and large cells must exhibit the immune-phenotype of follicle centre cells (pan-B antigen expression, CD-10+, BCL-2+, BCL-6+); (2) the growth pattern must be entirely diffuse.